![]() Gastric cancer is both common in China and the other parts of the world, and chemoprevention is always used as the main treatment for advanced cancer so far, which has become a focus topic in this area. ![]() The exact mechanisms of apoptosis are still unclear, but our earlier studies indicated that CLA can induce apoptosis in human gastric cancer SGC-7901 cells. Apoptosis, namely, programmed cell death, is an active and physiological process characterized by a series of morphological and biological alterations in which the cells become smaller, shrinking, the nuclei round up, the chromatin becomes agglutinated and marginated, the nuclear membrane breaks down, and followed by the degenerative changes of the cells. In many instances, growth inhibition following terminal differentiation or anticancer drug treatment results in apoptosis. This is because CLA was shown to inhibit in vitro the proliferation of human gastric cancer cells(SGC-7901), mammary cancer cells(MCF-7), of human malignant melanoma cells, colorectal cancer cells, and rat hepatoma cells and in animal studies to prevent the development of mouse epidermal carcinogenesis, mouse forestomach cancer and of rat mammary tumorigenesis.Īlthough the exact mechanisms are not clear, the inhibitory effect of CLA on the proliferation of rapidly dividing cells has been attributed to the induction of cell cycle arrest and the induction of apoptosis. In recent years, CLA has received considerable attention as a chemopreventive agent. The biosynthesis of CLA in ruminants is the result of a rumen bacterium, which is known to convert linoleic acid to stearic acid via CLA. But expression of c-myc on SGC-7901 cells is lower than that in negative control, which needs to be studied further.Ĭonjugated linoleic acid(CLA), a derivative of a fatty acid linoleic acid (LA), is a minor fatty acid found especially in red meat and in dairy products. Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations for various time periods significantly decreased the expressions of ki67 (the expression rates were 18.70%-3.20%, at 24 h and 8.10%-0.20% at 48 h, respectively), bcl-2 (4.30%-0.15% at 24 h and 8.05%-0% at 48 h),and c-myc (4.85%-2.20% at 24 h and 4.75%-0.30% at 48 h) as compared with those in the controls (the expressions of ki67, bcl-2, and c-myc were 15.1% at 24 h and 13.5% at 48 h, 6.80% at 24 h and 8.00% at 48 h, 5.50% at 24 h and 5.30% at 48 h, respectively) ( P < 0.01), whereas the expressions of Fas were increased (0.60%-2.75%, 24 h and 0.45%-5.95%, 48 h).ĬONCLUSION: The growth and proliferation of SGC-7901 cells are inhibited by c9, t11-CLA via blocking the cell cycle, pathways of bcl-2-associated mitochondria with reduced expression of bcl-2 and Fas-associated death domain protein (FADD) with enhanced expression of Fas. To further investigate the influence of the cell cycle progression, we found that apoptosis induced by c9, t11-CLA may be involved in blocking the cell cycle of SGC-7901 cells.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |